Fig. 7

RB1 is involved in ICCG gene regulation. a mRNA expression levels of the four ICCG genes were evaluated by RT-qPCR in SAOS-2 cells transfected with a vector coding for the RB1 protein (pRB) or with a vector coding for a truncated and non-functional protein (pRBΔ). Data were normalized with ACTINB expression levels and are expressed relative to the levels found in the pRBΔ cells. Values represent the mean (± sem) of at least three independent experiments. *p < 0.05, ***p < 0.001. b Analysis for the presence of 165 previously characterized RB1-regulated genes [34] among PCCG and ICCG genes. Histograms represent the ratio between observed and expected matches, in a base 2 logarithmic scale. Statistical analysis was obtained by a Fisher test comparing observed vs. expected matches. *p < 0.05. c Western-blot was performed to analyze RB1 protein expression in BJ-hTERT-derived clones, either with or without 7 days exposure to doxycycline. pKu80 protein was used as a loading control