Fig. 6

Activation of CG genes and down-regulation of ICCG genes persist past a transient phase of DNMT1 depletion. a Schematic outline of the experiment. BJ-hTERT-pTshDNMT1 cells were cultured in the absence (ctrl) or in the presence of doxycycline (doxy) for 7 days (d7), and thereafter, in normal medium during seven more days (d7 + 7). Control and post-doxy cell populations were then cloned by limiting dilution. RNA was extracted from clones between day 36 and 82. b DNMT1 expression levels were analyzed by RT-qPCR in control and treated cell populations at the indicated time points. Data, which derived from at least four independent experiments, were normalized with ACTINB expression level and are expressed relative to the levels found in control cells. **p < 0.01. c Expression levels of the CG gene MAGEA1, the four ICCG genes, and the three non-ICCG genes were assessed by RT-qPCR in control and post-doxy clones. Red dots correspond to clones showing activation of MAGEA1. Black bars correspond to the mean. **p < 0.01, ***p < 0.001, ****p < 0.0001