Fig. 2

CG gene activation is associated with DNMT1 down-regulation in melanoma tissues. a The 45-MelCells dataset was analyzed for the expression of seven DNA methylation-associated genes (DNMT1, DNMT3A, DNMT3B, UHRF1, TET1, TET2, and TET3) and of the four representative ICCG genes. Graphs represent relative probe intensities for each gene (in a log2 scale), and each dot corresponds to one cell line. Cell lines were separated into two groups according to their CGAS (≤2 and ≥7). Differential gene expression between the two groups were evaluated (Mann–Whitney tests), and genes showing a significant p value are shown in bold (*p < 0.05, **p < 0.01). Red bars represent the median. b A similar analysis was performed in a dataset of melanoma tissue samples (TCGA). Samples were grouped according to their CGAS: CGAS-low and CGAS-high correspond to the lower and upper quartile, respectively (n = 70 in each group). P values were obtained as described here above. *p < 0.05, **p < 0.01, ***p < 0.001. c Analysis of immunohistochemistry (IHC)-based protein expression data in melanoma tissues (Human Protein Atlas). Melanoma samples where IHC data were available for both DNMT1 and CG protein levels are presented