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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Analysis of DNA methylation landscape reveals the roles of DNA methylation in the regulation of drug metabolizing enzymes

Fig. 5

Validation of mRNA expression and DNA methylation of the CYP1B1 gene in hepatoma cells. a The level of DNA methylation was mapped on the CYP1B1 gene locus in the three hepatoma cell lines. The gene structure and location of the CpG sites are shown in the lower panel. b The levels of CYP1B1 mRNA were detected by quantitative real-time PCR in hepatoma cells treated with TSA and/or DAC. The vertical axis indicates the mRNA level of treated cells relative to paired control cells without treatment. Each column represents the mean ± SD (n = 3). c The CYP1B1 gene methylation status of the three control cell lines and adult liver tissues was examined by COBRA assay. DNA fragments cleaved by TaqI digestion represent methylated DNA (M), while noncleaved fragments represent unmethylated DNA (U)

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