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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Nucleated red blood cells impact DNA methylation and expression analyses of cord blood hematopoietic cells

Fig. 3

DNAm profiles of cord blood cells isolated using the stringent FACS strategy. a Unsupervised Euclidean clustering of genome-wide DNAm (440,315 CpG sites) in CD4 and CD8 T cells (CD4T and CD8T, respectively), monocytes (Mo), and nRBCs isolated by a stringent FACS protocol; letters in the sample labels indicate different cord blood donors. b Number of large magnitude DM sites (FDR <5 %, |Δβ| >0.20) between nRBCs and CD4 T cells, nRBCs and monocytes, and CD4 T cells and monocytes sorted using a stringent approach. c–e Overlap of cell-specific DM sites (FDR <5 %, |Δβ| >0.20) identified in the standard versus stringent sorting protocols for c T cells, d monocytes, and e nRBCs. Note that the stringent protocol identified a large number of additional DM sites in T cells and that the nRBC DM sites found by the two sorting protocols showed more differences than similarities

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