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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Global analysis of DNA methylation in hepatocellular carcinoma by a liquid hybridization capture-based bisulfite sequencing approach

Fig. 5

Flow chart. Eight pairs of HCC samples were used to screen for candidate methylation markers using the promoter-targeted LHC-BS approach. In HCC tissues, 2972 DMRs were determined and 77 genes with one or two DMRs were found to be common in 6 of 8 paired samples. Gene expression was analyzed using the Illumina high-throughput RNA-seq technology, and 7019 DEGs were detected. Among them, 93 DEGs were shared by 6 of the 8 paired samples. Through cross-matching DMR-containing genes with DEGs and searching the literature, 20 genes were selected for validation. Twelve candidate genes were validated for methylation and gene expression in 78 paired HCC samples. Functional validation was performed in vitro, and 7 genes were identified as candidate genes in HCC, whose altered expression may contribute to hepatocarcinogenesis

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