Detection of nasopharyngeal carcinoma in Morocco (North Africa) using a multiplex methylation-specific PCR biomarker assay

Table 6 Sequence of MSP primers used in the study

Marker		Primer sequence (5′–3′)	Size (bp)	Refs
EBNA1	F	AAGAGGTTTAGGAGTTTTAGTAGTTAGTTAT	130	^a
EBNA1	R	CACCTTCTTAATAATATTCAAAATAATC	130	^a
U-LMP1	F	GGGGGGATTTGTTTTTTTAATATAAATATAT	145	^a
U-LMP1	R	TAAAATATAAACCCAAAAAAATTTACA	145	^a
M-ITGA9	F	GTTGTTGGTTCGGAGATTATATTTC	230	^a
M-ITGA9	R	AAAACAACCCGAATAAAAAACG	230	^a
M-RASSF1A	F	GGGTTTTGCGAGAGCGCG	169	[79]
M-RASSF1A	R	GCTAACAAACGCGAACCG	169	[79]
M-P16	F	CGAGTATTCGTTTACGGC	106	^a
M-P16	R	CTTCCTCCGATACTAACG	106	^a
M-DAPK	F	GGATAGTCGGATCGAGTTAACGTC	98	[79]
M-DAPK	R	CCCTCCCAAACGCCGA	98	[79]
M-CHFR	F	GTTTTAATATAATATGGCGTCGATC	213	^a
M-CHFR	R	CTCAACTAATCCGCGAAACG	213	^a
M-GAPDH	F	TTAGGTGGTTTTTTTTGATTTTAAT	192	^a
M-GAPDH	R	AAATTATCAAAACCCTTTTTCTAAACCAA	192	^a
M-WNT7A	F	GTAGTTCGGCGTCGTTTTAC	123	[80]
M-WNT7A	R	CGAAACCGTCTATCGATACG	123	[80]
M-CYB5R2	F	GGGGAGCGGGTTAGTCGTC	140	[81]
M-CYB5R2	R	GAACCCGCAAACTCGTAACGTC	140	[81]
M-WIF1	F	GGGCGTTTTATTGGGCGTATC	198	^a
M-WIF1	R	TAACGAAACCAACAATCAACG	198	^a
M-RIZ1	F	ATTTTAGTTTTAGGGTGCGGTC	233	^a
M-RIZ1	R	AACTCCAATCGAAAATAACGTC	233	^a
M-FSTL1	F	TCGAGGTTGGCGATCGCG	171	[82]
M-FSTL1	R	CGCAAACTCGCTCCGACCG	171	[82]

ISSN: 1868-7083