Table 1 Associations between GDM and methylation of candidate regions assayed by pyrosequencing in validation cohort (RICHS) and verification set (HEBC)
A. Locus closest to candidate on microarraya | |||||
| Â | Association with GDM [coef (95Â % CI)] | ||||
| Â | Gene in proximity | CpG ID | 450K array (beta) | Validation set (%) | Verification set (%) |
|  |   HLA-DOA | cg08147094 | −0.12 (−0.18, −0.06) | −3.24 (−7.93, 1.44) | −13.95* (−20.50, −7.40) |
|  |   HLA-H/HLA-J | cg23681866 | 0.11 (0.05, 0.17) | −1.96 (−7.46, 3.54) | 6.21 (−0.18, 12.59) |
|  |   SNRPN/SNURF | cg18506672 | −0.09 (−0.13, −0.04) | −0.49 (−2.41, 1.43) | −7.24* (−11.44, −3.04) |
|  |   CCDC181 | cg25464921 | 0.14 (0.07, 0.21) | −3.28 (−7.90, 1.34) | 9.02* (3.53, 14.52) |
B. Regional change within pyrosequenced regionb | |||||
| Â | Association with GDM [coef (95Â % CI)] | ||||
| Â | Gene in proximity | Size of region (Candidate) | 450K array (beta) | Validation set (%) | Verification set (%) |
|  |   HLA-DOA | 2 (CpG 1) | – | −3.01 (−6.17, 0.16) | −9.23* (−13.67, −4.79) |
|  |   HLA-H/HLA-J | 3 (CpG 2) | – | −1.56 (−6.12, 3.01) | 4.82 (−0.78, 10.42) |
|  |   SNRPN/SNURF | 2 (CpG 1) | – | −0.44 (−4.70, 3.82) | −4.70 (−10.63, 1.23) |
|  |   CCDC181 | 8 (CpG 6) | – | −2.42 (−6.89, 2.04) | 9.34* (3.41, 15.27) |