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Fig. 2 | Clinical Epigenetics

Fig. 2

From: MicroRNA-101 is repressed by EZH2 and its restoration inhibits tumorigenic features in embryonal rhabdomyosarcoma

Fig. 2

Inhibition of EZH2 restores endogenous miR-101 levels in eRMS cells. a RT-qPCR analysis of mature forms of miR-214, miR-29b, and miR-101 in RD, JR1, and RD18 cells 72 h post EZH2 siRNA transfection (RD were transfected with SMART pool siRNA EZH2 (asterisks), JR1 and RD18 were transfected with siRNA targeting 5′-UTR of EZH2, see “Methods” section). Data normalized using snoU6 and expressed as fold increase over a non-targeting siRNA (CTR siRNA, 1 arbitrary unit). Columns, means; bars, SD. Results from three independent experiments are shown. *P < 0.05 (Student’s t-test). b MiR-101 level in RD, JR1, and RD18 cells daily treated for 72 h with either S-adenosyl-L-homocysteine hydrolase inhibitor 3-deazaneplanocin A (DZNep) (5 μM) or vehicle (i.e., water, referred as untreated condition: UN), normalized using snoU6 and expressed as fold increase over UN (1 arbitrary unit). Columns, means; bars, SD. Results from three independent experiments are shown. *P < 0.05 (Student’s t-test)

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