Fig. 5From: Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputsPerformance of gene-specific assay for 100, 50, 25, 5, and 0 % methylated DNA samples. a Gel electrophoresis images of RPA amplifications and photo of HRP/TMB reactions. Top: post-MBD enrichment. Bottom: input controls i.e., before MBD enrichment. b Corresponding absorbance measurements. Blue: post-MBD enrichment. Red: input control. c Calibration plot of HRP/TMB response to methylation changes. Error bars represent SD, n = 3Back to article page