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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs

Fig. 1

Assay schemes. a Strategy for total genomic methylation. Genomic DNA is restriction enzyme (RE) digested, enzymatically biotinylated via a fill-in reaction with Klenow polymerase and biotin-dNTPs. MBD magnetic beads are then used to select for methylated DNA. Colorimetric evaluation is mediated by SA-HRP which recognizes the biotin on enriched methylated DNA. b Strategy for gene-specific methylation. Genomic DNA is RE digested and methylated DNA is selected via MBD enrichment. Gene-specific isothermal amplification is then performed with biotin-dNTP to generate biotin-DNA polymers which are in turn selected for with SA magnetic beads and SA-HRP for colorimetric evaluation

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Clinical Epigenetics

ISSN: 1868-7083

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