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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Genetic and epigenetic alterations of netrin-1 receptors in gastric cancer with chromosomal instability

Fig. 3

UNC5C promoter methylation and 4q LOH analyses. (a) Schematic representation of the location of the three LOH probes and UNC5C gene promoter regions in chromosome 4. The red line denotes the UNC5C gene. Gray and black squares represent the untranslated and the coding exon 1 regions, respectively; arrows on the squares indicate transcriptional starting sites; vertical lines indicate CpG sites; white diamonds represent the restriction sites for HhaI; thick horizontal lines depict the location of COBRA products; arrows on the thick horizontal lines denote COBRA primers. (b) Representative results of COBRA of UNC5C. Arrows indicate methylated alleles; M denotes methylation; U denotes unmethylation; Mc denotes the methylated control; SM denotes the size marker. (c) Results of UNC5C methylation as a continuous variable. In the box plot diagrams, the horizontal line within each box represents the median, the limits of each box represent the interquartile ranges, and the whiskers denote the maximum and minimum values. Each green bar represents the mean. NM denotes normal mucosa. T denotes tumor. (d) The frequency of methylation-positivity for cancer and normal tissues according to different thresholds. (e) UNC5C mRNA expression levels and methylation status in 10 gastric cancer cell lines and a human lung fibroblast cell line. UNC5C mRNA expression is observed (lower ΔCT) in GCIY and NHLH cell lines. DW denotes distilled water

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