Fig. 3

Loss of CCDC8, BNC1 or GALNT9 expression increases the migratory potential of breast cancer cell lines. Breast cancer cell lines that expressed CCDC8, BNC1 or GALNT9 were identified; the expression of these genes was knocked down by siRNA (see Additional file 11: Figure S7 and Additonal file 12: Fig. S8). a T47D cell lines transfected with siRNA oligos against CCDC8; b MCF7 cell lines transfected with siRNA oligos against BNC1 or c MDA-MB231 cell lines transfected with siRNA against GALNT9 exhibited more migratory potential compared to respective cell lines transfected with control siRNA oligos. Following siRNA transfection, confluent cells were incubated in serum-free media and an artificial wound was scratched through them (0 h). Forty eight hours later the distance migrated was calculated by subtracting the value of non-migrated distance from the initial would. The distance migrated (in percentage) by respective cell lines knocked down with siRNA against CCDC8, BNC1 or GALNT9 in compared to the respective cell lines transfected with control siRNA oligos was statistically significant (p = 0.001, 0.011 and 0.027, respectively)