Table 1 Differentially methylated genes as revealed by genome-wide methylation analysis, which were selected for validation by pyrosequencing and RT-qPCR
Genes | MCIp-chip | Pyrosequencing | Expression (RT-qPCR) |
|---|---|---|---|
RSPO2 | • | • | Underexpressed |
TWIST1 | • | • | Underexpressed |
SOX3* | • | • | Underexpressed |
SOX17 | • | • | Underexpressed |
PROM1/CD133 | • | • | Underexpressed |
HOXA3* | • | • | Underexpressed |
MEIS1 | • | • | Underexpressed |
OTX2 | • | • | Underexpressed |
SOX14 | • | • | N.S. |
ONECUT1 | • | • | N.S. |
PAX7 | • | • | N.S. |
FOXA1 | • | • | N.S. |
LHX5 | • | • | - |
BDNF | • | • | - |
NKX2.2 | • | • | - |
NKX2.3 | • | • | - |
CDX2 | • | • | - |
ONECUT2 | • | • | - |
LIN28A | • | • | - |
WIT1 | • | • | - |