Identification of hypomethylation at the cardinal loci in an MLMD sample. Upper panel: Genomic location from the UCSC genome browser, illustrating the KCNQ1 gene and the imprinting control region. Lower panel: graphical presentation of 450 k DNA methylation data across the KCNQ1 gene in BWS-MLMD 4. The x-axis corresponds to the genomic location as illustrated in the upper panel. The primary y-axis (left) represents the CH P value (solid blue line); the secondary y-axis (right) represents the difference in M value between BWS-MLMD 4 and controls (dashed black line). BWS-MLMD, Beckwith-Wiedemann syndrome-multi-locus methylation disorders.