Figure 7

The relationship between B cell activity and miR-1246. We treated three healthy B cells with PBS, anti-IgM and anti-CD40 antibodies, or AKT inhibitor pre-treated + anti-IgM and anti-CD40 antibodies. (A-C) Increased AKT protein phosphorylation and decreased P53 protein and miR-1246 expression in anti-IgM and anti-CD40 antibodies-treated healthy B cells, compared with PBS-treated healthy B cells. Decreased AKT protein phosphorylation and increased P53 protein and miR-1246 expression in pre-treated AKT inhibitor + anti-IgM and anti-CD40 antibodies-treated healthy B cells compared with anti-IgM and anti-CD40 antibodies-treated healthy B cells. The Western blot image is a representative image. Data are presented as the mean ± SD of the same experiments performed in three healthy donors. (*P < 0.05; **P < 0.01).