The relationship between B cell activity and miR-1246. (A-E) MiR-1246 expression (A), early B cell factor 1 (EBF1) protein level, AKT phosphorylation and P53 protein level (B, C), percentage (D), and mean fluorescence intensity (MFI) (E) of CD40, CD80, and CD86 in stimulated B cell transfected with miR-1246 mimic and mimic control. CD4+T cells which were isolated from the same healthy control were cultured in RPMI 1640 medium with 10% FBS, 100 U/ml penicillin G, and streptomycin. After stimulation and transfection, B cells were co-cultured with autologous CD4+T cells at a ratio of 4:1 in 96-well round-bottomed plates. At 24 h, percentage (F) and MFI (G) of CD40L, CD28, and CD152 were measured by flow cytometry. The Western blot image is a representative image. Data are presented as the mean ± SD of the same experiments performed in three healthy donors. (*P < 0.05; **P < 0.01).