The relationship between B cell activity and miR-1246. (A) Measurement of miR-1246 level after stimulated with anti-IgM and anti-CD40 antibodies or PBS (control) in normal B cells. (B, C) Representative Western blotting results and densitometric analysis for early B cell factor 1 (EBF1) protein level, AKT phosphorylation, and P53 protein level in normal B cells after stimulated with anti-IgM and anti-CD40 antibodies and PBS (control). (D, E) Percentage (D) and mean fluorescence intensity (MFI) (E) of CD40, CD80, and CD86 in normal B cells stimulated with anti-IgM, anti-CD40, and PBS (control). Data are presented as the mean ± SD of the same experiments performed in three healthy donors. (*P < 0.05; **P < 0.01).