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Figure 4 | Clinical Epigenetics

Figure 4

From: The role of microRNA-1246 in the regulation of B cell activation and the pathogenesis of systemic lupus erythematosus

Figure 4

MiR-1246 represses B cell responsiveness. (A, B) Inhibiting miR-1246 expression in healthy controls’ B cells increases CD40, CD80, and CD86 expression and enhances B cell responsiveness. All panels, normal B cells were transfected with miR-1246 inhibitor or inhibitor control. Transfected cells were stained with PE-Cy7-conjugated anti-human CD40, FITC-conjugated anti-human CD80, PerCP-Cy5.5-conjugated anti-human CD86, and APC-conjugated anti-human CD19 and analyzed by flow cytometry, percentage (A), and mean fluorescence intensity (MFI) (B) for CD40, CD80, and CD86 in normal B cells inhibiting miR-1246 and in inhibitor controls (*P < 0.05). (C, D) Overexpression of miR-1246 in SLE B cells decreases CD40, CD80, and CD86 expression and reduces B cell responsiveness. All panels, SLE B cells were transfected with miR-1246 mimic or mimic control. Transfected cells were stained with PE-Cy7-conjugated anti-human CD40, FITC-conjugated anti-human CD80, PerCP-Cy5.5-conjugated anti-human CD86, and APC-conjugated anti-human CD19 and analyzed by flow cytometry, percentage (C), and MFI (D) for CD40, CD80, and CD86 in SLE B cells overexpressing miR-1246 and in mimic controls (*P < 0.05). All data represent the mean ± SD results of three independent experiments. All experiments were performed in triplicate.

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