Identification of miR-1246 target mRNAs in systemic lupus erythematosus (SLE) B cells. (A, B) Early B cell factor 1 (EBF1) protein level in B cells from active SLE patients (n = 30) and healthy controls (n = 20). EBF1 expression was significantly increased in active SLE B cells compared with that in control B cells. Representative Western blotting and quantitative analysis of the band intensities of control (n = 4) and SLE (n = 4) samples normalized to β-actin is shown at the bottom. (C) In B cells derived from active patients with SLE, has-miR-1246 transcript levels were negatively correlated with the levels of EBF1 protein (r = −0.82, P < 0.01, n = 30). (**P < 0.01).