Figure 6From: Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients The methylation level in each cytosine in sperm DNA was analyzed by pyrosequencing. (A) DNA methylation in 12 cytosines (CpG) in H4K12ac-associated NSD1 promoter in fertile donors and subfertile patients. (B) Averaged DNA methylation in 1 to 4 CpGs and 5 to 12 CpGs of NSD1 promoter in fertile and subfertile men. (C) DNA hypermethylation in 12 CpGs in sperm-specific protamine promoter. (D) Averaged methylation from 12 CpGs in protamine promoters showing no significant difference between fertile and subfertile sperm.Back to article page