Figure 4
Pyrograms showing methylation level for each cytosine within the investigated sequences in spermatozoa of one fertile donor and two selected infertile patients. In contrast to the hypomethylated promoter region of NSD1, paternally imprinted and hypermethylated H19 locus is presented as a control. Pyrosequencing using fully methylated and fully unmethylated DNA was carried out for the internal control.