Validation of enriched promoters with μChIP in combination with real-time polymerase chain reaction (PCR) between sperm of fertile donors and subfertile patients. Results from ChIP assay with anti-H4K12ac antibodies, with anti-protamine-1 in immunoprecipitated DNA of fertile donors (n = 5) and subfertile patients (n = 8). Unmodified H3 was used as a positive and IgG as a negative isotype control. Loss of interaction for H4K12ac was detected in selected promoters; however, a significant difference was observed in NCOA6, RUVBL1, and POU2F1. Depletion of H4K12ac binding is potentially replaced by either unmodified histone H3 or protamine 1.