Skip to main content
Figure 1 | Clinical Epigenetics

Figure 1

From: Replicative senescence is associated with nuclear reorganization and with DNA methylation at specific transcription factor binding sites

Figure 1

Telomere distribution in senescent fibroblasts. Telomeres were analyzed by Q-Fish (labeled with Cy3) in nuclei of fibroblasts of early or late passage (n = 3). The nuclear region was counterstained with DAPI. An overview of a cytospin (A) and enlarged nuclei at early and late passage (B) are exemplarily depicted. Separation of nuclear zones in the border, middle, and center is indicated by yellow, white, and violet lines, respectively (size bar = 5 μm). Overall, the nuclear area was greatly increased in cells of late passage (C) and nuclei became more elongated (D). Telomere length markedly decreased in fibroblasts of late passage (E) (a.u. = arbitrary units; error bars depict standard error of nuclei analyzed; early passage: 374 nuclei; late passage: 151 nuclei). The distribution of telomeres changed upon senescence: in early passages (purple dots), they were primarily localized in border and middle regions, whereas distribution changed towards the nuclear center in late passages (yellow dots, data from three biological replica, t-test in all statistical analyses) (F).

Back to article page