DNA methylation at particular CG dinucleotides within the human PPARα gene promoter (A), PPARδ gene promoter (B), TGFβ1 exon 1 (C), collagen 1A1 intron 1 (D) and PDGFα gene promoter (E) in liver biopsy tissues from patients with mild (0 to 2 Kleiner score) or severe (3 to 4 Kleiner score) NAFLD was determined by pyrosequencing. The relative position and surrounding sequence of the differentially methylated CGs are shown in the schematic drawing above the graphs. Differences are expressed as percentage of DNA methylation. Error bars represent mean values ± standard error of the mean (SEM). *
P < 0.05; **
P < 0.01.