Figure 4From: Aberrant DNA methylation of imprinted loci in hepatocellular carcinoma and after in vitro exposure to common risk factors In vitro models of risk factor exposure. Primary human hepatocytes were naturally infected with HBV. Efficiency was monitored by qRT-PCR of HBx transcript (upper panel). DNA extracted at different time points was used for pyrosequencing analysis of selected imprinted regions comparing mock to HBV-infected hepatocytes (lower panels).Back to article page