Deregulation of the 15q11-13 cluster in HCC. (A) Pyrosequencing assays to assess Tumor vs. Surrounding methylation at imprinting control regions (ICR) for MEST and KCNQ1 (n = 5 pairs). The ICR for the 15q11-13 cluster, composed of the Prader-Willi Syndrome region (PWS-SRO) and the Angelman Syndrome region (AS-SRO), was also assessed. (B) Diagram of the 15q11-13 cluster showing the different regions of study, including the ICR and two control non-imprinted (ni) genes (APBA2 and TJP1). (C) qRT-PCR for selected genes in the 15q11-13 cluster comparing tumors and adjacent tissues (n = 5 tumor/surrounding pairs). *P value <0.05.