Technical validation of a set of DM CpG sites using pyrosequencing. (A) The overall correlation plot between the Beta values of seven DM CpG sites obtained from Illumina HumanMethylation450 BeadChip array (y-axis) and methylation percentage from pyrosequencing (x-axis). Five case and five control samples for each CpG site were selected for pyrosequencing validation. (B, C, D, E, F, G, H) Bar plots to compare the average Beta values and pyrosequencing methylation percentage for individual CpG sites in (A): (B) cg26478401, (C) cg26076905, (D) cg11671363, (E) cg06111285, (F) cg06652329, (G) cg08198483, and (H) cg08537847. Values are presented as mean ± standard deviation (s.d.). The CpG sites that have significant P < 0.05 between cases and controls are labeled by (*).