Strength of associations of genome-wide autosomal CpG methylation status with age in our TFSE cohort. Manhattan plot shows the significance level of age association of each CpG locus by chromosomal position. Each grey dot represents an individual CpG site. The red line depicts the genome-wide significance threshold after correction for Bonferroni multiple testing, P
α=0.05 = 3.65 × 10−7. Colored dots represent epigenetic regions belonging to genes previously associated with obesity, T2D, and MetS. Blue dots obesity-related genes in adults, red dots obesity related genes in children, orange dots obesity related genes in children and adults, green dots T2D genes, yellow dots MetS genes. Selected genes are labeled. Genes found to be within an aDMC are shown in bold and italicized.