Figure 2

Validation of lncRNA dysregulation. The expression of 13 target lncRNAs in renal cell carcinoma (red) and normal renal (green) tissue was validated using quantitative real-time PCR; the expression levels were normalized using ACTB and PPIA as reference genes. We confirmed significant (all p < 0.001) overexpression of lnc-BMP2-2 (B), lnc-CPN2-1 (C), lnc-FZD1-2 (F), lnc-ITPR2-3 (G), lnc-SLC30A6-1 (K), and lnc-SPAM1-6 (L) in renal cell carcinoma, whereas lnc-ACACA-1 (A), lnc-FOXG1-2 (E), lnc-LCP2-2 (H), lnc-RP3-368B9 (I), and lnc-TTC34-3 (M) were significantly downregulated (all p < 0.001); lnc-ERCC5-1 (D, p = 0.401) and lnc-RP11-480I12.4.1-1 (J, p = 0.731) were not different in malignant and normal renal tissue. Receiver operator characteristic (N) analyses demonstrate excellent discrimination of the lncRNAs between both cohorts (area under the curve 0.90–0.94).