Figure 5From: Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting s-adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes Effect of vitamin B 12 on methylation index. AdoMet and AdoHcy was measured in cell pellets collected from the adipocytes cultured in customised media, control, LB, and NoB. (A) AdoMet, (B) AdoHcy, and (C) AdoMet-to-AdoHcy ratio. Normalized for cell number and expressed as pmol per 1 × 106 cells. All experiments were performed as triplicates. Values are mean ± SEM. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, P value compared to control.Back to article page