Effect of vitamin B
on methylation of cholesterol regulatory genes. Genome-wide DNA methylation analysis of cholesterol regulatory genes was performed in adipocytes cultured in customised media, control, LB, and NoB. (A) SREBF-1 and (B) LDLR from Illumina. Validation by BS-pyrosequencing was also done in the same samples and methylation status confirmed for (C) SREBF-1 and (D) LDLR. All experiments were performed as triplicates. Values are mean ± SEM. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, P value compared to control.