Effect of vitamin B
on cholesterol biosynthesis. (A) Total cholesterol from adipocytes cultured in customised media: control (500 nM B12), LB (0.15 nM B12), and NoB (0 nM B12). Total cholesterol was normalized for protein content and expressed as percentage. mRNA expression of cholesterol biosynthesis genes in adipocytes. (B) HMGCS1, (C) HMGCR, (D) IDI1, (E) SQLE, and (F) SC4MOL. All experiments were performed as triplicates. Values are mean ± SEM. *P ≤ 0.05; **P ≤ 0.01, ***P ≤ 0.001, P value compared to control.