DNA methylation is associated with poor survival in MT1 gene cluster regardless of ERα
status of patients. (A) Kaplan-Meier survival curves indicating that MT1 gene cluster hypermethylation is associated with decreased survival in both ERα + and ERα − patients. (B) A table showing the hazard ratios along with 95% confidence intervals and statistical significance for methylation along with other covariates. The groups are the same as the ones used for Figure 4A and MT1 cluster in Figure 2B, that is, patients showing hypermethylation in one to nine MT1 genes in the cluster are grouped together. (C) A heat map showing methylation of MT1 gene cluster for normal breast tissue, breast tumors, and breast cancer cell lines. Dashed-line squares highlight differentially methylated regions in these samples. Note that MT1 gene cluster consists of nine loci (MT1L, E, M, A, B, F, G, H, and X) and two pseudogenes (MT1DP and IP) as outlined in the genomic map above the methylation profile.