DNA hypermethylation in breast cancer samples relative to normal breast tissue in promoter and non-promoter CpG islands. Methyl capture sequencing (MBD-seq) was used to generate DNA methylation profiles of the genomes of breast tumors (n = 77) and normal breast tissue (n = 10). (A) Pie charts demonstrate differential methylation in promoter, intragenic, and intergenic CGIs as well as non-CGI promoter regions (left panel). Methylation of core and shore regions is also demonstrated under each corresponding CGI type (left panel). DNA hypermethylation in core and shore regions are shown for breast tumors compared to normal tissue (right panel). (B) Example loci showing promoter CGI, intragenic, intergenic, and non-CGI promoter regions. Dashed squares highlight regions corresponding to breast cancer hypermethylation.