Table 2 Oligonucleotides used in this study
Name | Sequence 5′ to 3′ | Purpose |
|---|---|---|
NOS3_P1+ | tcagcctgcccctgaaac | (h)MeDIP/ChIP-qPCR |
NOS3_P1− | catccgtgggaacttgaaat | (h)MeDIP/ChIP-qPCR |
NOS3_P2+ | atttgtggggaaatcaaacg | (h)MeDIP/ChIP-qPCR |
NOS3_P2− | cccttcttgagacagccaca | (h)MeDIP/ChIP-qPCR |
NOS3_P3+ | attgtgctgaaatcgctcct | (h)MeDIP/ChIP-qPCR |
NOS3_P3− | agtaccccacccttgcacta | (h)MeDIP/ChIP-qPCR |
NOS3_P4+ | agaagaagggcctcacatca | (h)MeDIP/ChIP-qPCR |
NOS3_P4− | tcctgagtcacctgttctgtg | (h)MeDIP/ChIP-qPCR |
NOS3_P4a+ | cacaagactccagggaagca | (h)MeDIP/ChIP-qPCR |
NOS3_P4a− | ctgcagaaggtgctggtgg | (h)MeDIP/ChIP-qPCR |
NOS3_P5+ | ctggaatcccagcccatt | (h)MeDIP/ChIP-qPCR |
NOS3_P5− | cctcccggagtgattctcat | (h)MeDIP/ChIP-qPCR |
NOS3_P6+ | gctcccacttatcagcctca | (h)MeDIP/ChIP-qPCR |
NOS3_P6− | agccctggccttttccttag | (h)MeDIP/ChIP-qPCR |
NOS3_P7+ | gggggagatccttgcctttt | (h)MeDIP/ChIP-qPCR |
NOS3_P7− | ccaggtcttcactactgggc | (h)MeDIP/ChIP-qPCR |
NOS3_cDNA+ | tccacatgagctggggtaggca | cDNA/qPCR |
NOS3_cDNA− | gggactgtgccagatgttaggaga | cDNA/qPCR |
STAT3+ | gacattcccaaggaggaggc | cDNA/qPCR |
STAT3− | ggtcttcaggtatggggcag | cDNA/qPCR |
STAT3alpha+ | caggtagcgctgccccatac | cDNA/qPCR |
STAT3alpha− | atggtattgctgcaggtcgtt | cDNA/qPCR |
STAT3beta+ | gagccaggagcatcctgaag | cDNA/qPCR |
STAT3beta− | tccaaactgcatcaatgaatggt | cDNA/qPCR |
ATP6+ | gccctagcccacttcttacc | HKG |
ATP6− | ccagggctattggttgaatg | HKG |
BACT+ | gtcgacaacggctccggcatg | HKG |
BACT− | atgtcgtcccagttggtgacg | HKG |
GAPDH+ | aggtcggagtcaacggattt | HKG |
GAPDH− | tggaagatggtgatgggattt | HKG |
PECAM1+ | tgcctaacaccgcaagggcaa | HKG |
PECAM1− | actctgtggaccccaggttgaga | HKG |
RPL19+ | atcgatcgccacatgtatca | HKG |
RPL19− | ctggtcagccaggagctt | HKG |
VIL1+ | gcctcgatggaagcaacaaaacct | HKG |
VIL1− | agggtactgctttacaaccacagc | HKG |
NOS3_intron5out+ | caggcacctaccagcttagg | Nested PCR/genotyping |
NOS3_intron5out− | ggaactggggtagcagtgg | Nested PCR/genotyping |
NOS3_intron5in+ | ggatccagtgggggaagc | Nested PCR/genotyping |
NOS3_intron5in− | ttctctcttgggggagaagc | Nested PCR/genotyping |
NOS3_intron5_antisense+ | aggctgctcctgctactgac | cDNA/qPCR |
NOS3_intron5_antisense− | ctggaggaggggaaagaagt | cDNA/qPCR |
siRNA_STAT3 | AAGGAGGAGGCAUUCGGAAAGUAdTdT | siRNA |
dTdTUUCCUCCUCCGUAAGCCUUUCAU | ||
siRNA_NOS3 | AAGAGUUAUAAGAUCCGCUUCdTdT | siRNA |
dTdTUUCUCAAUAUUCUAGGCGAAG | ||
Luci+ | tgcaaaagatcctcaacgtg | cDNA/qPCR |
Luci− | aatgggaagtcacgaaggtg | cDNA/qPCR |
27 nt-siRNA motif 1 | gaagucuagaccugcugcgggggugag | siRNA |
cuucagaucuggacgacgcccccacuc | ||
27 nt-siRNA motif 2 | gaagucuagaccugcugcaggggugag | siRNA |
cuucagaucuggacgacguccccacuc | ||
NOS3-2800+ | aaacaccccgcctcctaac | ChIP-qPCR |
NOS3-2800− | tgcatgcacagtcacaca | ChIP-qPCR |
NOS3-1554+ | cagccgaacaccaaatctcc | ChIP-qPCR |
NOS3-1554− | cagccctgccaagaatgatg | ChIP-qPCR |
NOS3-1250+ | ccctgtccagagagcattca | ChIP-qPCR |
NOS3-1250− | ttgggtttgaattggggcag | ChIP-qPCR |
NOS3-929+ | cctctccattgcctccagag | ChIP-qPCR |
NOS3-929− | gttcctctctgtggggatcc | ChIP-qPCR |
NOS3-804+ | cacacaggttcagagcacac | ChIP-qPCR |
NOS3-804− | atgatctctgggtggctgtc | ChIP-qPCR |
NOS3-620+ | gatcatggagaaggggacgt | ChIP-qPCR |
NOS3-620− | cattgcccgcagtattcctc | ChIP-qPCR |