Prediction of ALL subtypes by consensus CpG sites defined using ALL samples of known subtype. (A) The estimated subtype probability scores of the 546 patients used to design the classifier. Subtype probability scores are plotted along the horizontal axis. The scores range from 0 to 1, where a score >0.5 is considered a positive classification. The patients are color coded by subtype along the vertical axis. The proportions on the right side of the panel give the number of patients accurately classified by subtype. (B) Hierarchical clustering of 546 ALL samples of known subtype and 139 non-leukemic reference samples according to the methylation levels of the 232 autosomal consensus CpG sites. Samples are clustered along the horizontal axis and the consensus CpG sites are clustered along the vertical axis. In the heatmap, blue indicates low, yellow indicates intermediate, and red indicates high methylation levels.