Figure 3

Effects of ABCA1 knockdown on cholesterol level and ovarian cancer growth in vivo . The cholesterol level of ABCA1 knockdown (A) MCP3 and (B) HeyC2 cells was measured by cholesterol quantitation assay. (C) The effect of ABCA1 knockdown on tumor growth in vivo was determined by the nude mice model. HeyC2 cells stably infected with shABCA1 (red arrow) or shGFP (green arrow) were injected subcutaneously into athymic nude mice. One week later, tumor volumes were measured daily. From day 19, the volume of tumors with ABCA1 knockdown was significantly reduced as compared to vector controls (*P < 0.05; **P < 0.005). Data were expressed as mean ± SD (n = 3).