Nucleosome occupancy and endogenous CpG methylation at the HSPA5 gene. (A) Schematic showing the location of the NOMe-seq assay (black bar) in relation to the HSPA5 promoter. The arrow represents the transcriptional start site. (B) NOMe-seq was used to map the position of nucleosomes on individual DNA molecules at the HSPA5 gene in LCLs derived from the six individuals with splice site mutations in MLH1. Vertical black bars below line = GpC sites; vertical black bars above line = CpG sites; pink bars = the location of nucleosomes on individual DNA molecules as determined by GpC methyltransferase inaccessibility; black circles = methylated GpC/accessible to M.Cvi PI; white circles = unmethylated GpC/inaccessible to M.Cvi PI. All endogenous CpG sites were unmethylated and triangles are not shown to provide better clarity of GpC sites.