Skip to main content
Figure 6 | Clinical Epigenetics

Figure 6

From: Concomitant downregulation of the imprinted genes DLK1 and MEG3 at 14q32.2 by epigenetic mechanisms in urothelial carcinoma

Figure 6

Chromatin immunoprecipitation (ChIP) analyses of histone modifications at the DLK1 promoter, the IG differentially methylated region (DMR) and the MEG3 DMR. Results of quantitative reverse transcription polymerase chain reaction (RT-PCR) conducted using DNA after ChIP with antibodies against active and repressive histone modifications at the DLK1 promoter (left), IG DMR (center), MEG3 DMR (right) in HepG2 and uncultured primary urothelial cells as controls and seven urothelial carcinoma cell lines derived from tumors of different stages and grades, that is, papillary urothelial cancer cell lines (BC61, J82, SW1710) and invasive urothelial cancer cell lines (5637, RT112, 639v and T24). CTCFL was used as reference for a silenced gene with repressive histone marks (H3K9me3 and H3K27me3), whereas GAPDH was used as control for a highly expressed gene with associated active histone modifications (H3K4me3 and H4K16ac). The 100% enrichment refers to the levels at the respective control genes. An alternative representation of the Figure and additional ChIP control experiments are displayed in Additional file 1: Figure S4.

Back to article page