Figure 3

Gene copy number analysis of the 14q32 imprinting cluster. Gene copy numbers analysis by qPCR of the DLK1 promoter (blue squares), the IG DMR (green rhombi) and the MEG3 DMR (red triangles) in leukocytes, benign (BN) and cancerous (BT) bladder tissues, urothelial cancer cell lines and normal urothelial cells (UP). Copy numbers were normalized to those in leukocytes set as 2 and to GAPDH as a reference gene. The asterisks indicate samples analyzed for DNA methylation by bisulfite sequencing. All other samples were analyzed only by pyrosequencing.