Leucine alters hepatic glucose/lipid homeostasis via the myostatin-AMP-activated protein kinase pathway - potential implications for nonalcoholic fatty liver disease

Table 2 Primer sequences used in real-time PCR (qPCR)

Gene name	Primers	Primer sequences
*SLC2A2*	Sense	5'- CATTCCAATTAGAAAGAGAGAACGTC-3'
*SLC2A2*	Antisense	5'-AGCAAACCTGTTTATGCAACC-3'
*G6Pase*	Sense	5'-TACGTCCTCTTCCCCATCTG-3'
*G6Pase*	Antisense	5'-CCTGGTCCAGTCTCACAGGT-3'
*PCK1*	Sense	5'-GGTTCCCAGGGTGCATGAAA-3'
*PCK1*	Antisense	5'-CACGTAGGGTGAATCCGTCAG-3'-3'
PC	Sense	5'-TTGCCCACTTCAAGGACTTC-3'
PC	Antisense	5'-CTTTGATGTGCAGCGTCTTG-3'
*FOXO1*	Sense	5’-GCTGCATCCATGGACAACAACA-3'
*FOXO1*	Antisense	5’-CGAGGGCGAAATGTACTCCAGTT-3'
*PPARGC1A*	Sense	5'-TGTGCAACTCTCTGGAACTG-3'
*PPARGC1A*	Antisense	5'-TGAGGACTTGCTGAGTGGTG-3'
*MSTN*	Sense	5'-CGTCTGGAAACAGCTCCTAACA-3'
*MSTN*	Antisense	5'-GAAAATCAGACTCTGTAGGCATGGT-3'
*GPAT1*	Sense	5’-AACCCCAGTATCCCGTCTTT-3’
*GPAT1*	Antisense	5’-CAGTCACATTGGTGGCAAAC-3’
*FOXA2*	Sense	5-TGTTCATGCCGTTCATCCC-3
*FOXA2*	Antisense	5-GGAGCGGTGAAGATGGAAG-3
GK	Sense	5'-GATGCACTCAGAGATGTAGTCG-3'
GK	Antisense	5'-TGAAGGTGGGAGAAGGTGAG-3'
*ACC1*	Sense	5’-ATCCCG TACCTTCTTCTACTG-3’
*ACC1*	Antisense	5’-CCCAAACATAAGCCTTCACTG-3’
*PPAR* γ	Sense	5'-CCACTATGGAGTTCATGCTTGTGAAGG-3'
*PPAR* γ	Antisense	5'-TGCAGCGGGGTGATGTGTTTGAACTTG-3'
*PPIA*	Sense	5'-TCTTGAGGGAAGCATATTGG-3'
	Antisense	5'-CAGGGAGACTGACTGTAGCAC-3'

ISSN: 1868-7083