Expression of the genes involved in glucose and lipid sensing after leucine supplementation of HepG2 cells. (A) Real-time PCR (qPCR) and immunoblotting analysis of expression levels of genes involved in gluconeogenesis, glucose uptake and lipogenesis. (B) qPCR and immunoblotting analysis of specific upstream genes involved in glucose/lipid sensing. Western blotting was performed using cell lysates supplemented with leucine for 48 h. Values are presented as mean ± SEM. Statistical significance relative to untreated control, *P <0.05 (n =3).