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Figure 4 | Clinical Epigenetics

Figure 4

From: Hypomethylation and overexpression of ITGAL (CD11a) in CD4+ T cells in systemic sclerosis

Figure 4

CD11a overexpression in CD4+T cells increased proliferative response to autologous PBMCs and enhanced production of IgG by co - cultured B cells. (A) SSc CD4+ T cells significantly increased proliferative response to autologous PBMCs than normal CD4+ T cells. (B) 5-azaC-treated CD4+ T cells showed greater proliferative response to autologous PBMCs than untreated CD4+ T cells. (A and B) The neutralizing antibodies to CD11a markedly decreased proliferative response of SSc CD4+ T cells and 5-azaC-treated CD4+ T cells but its isotype control, IgG, did not. (C) The IgG production was significantly higher in B cells co-cultured with autologous CD4+ T cells from SSc patients than in normal CD4+ T cells. (D) Co-culture of untreated and 5-azaC-treated CD4+ T cells with autologous B cells demonstrated enhanced production of IgG by B cells co-cultured with 5-azaC-treated CD4+ T cells. (C and D) The IgG level yielded by B cells cultured alone was significantly lower than that by (C) B cells co-cultured with SSc CD4+ T cells or (D) B cells co-cultured with 5-azaC-treated CD4+ T cells. Anti-CD11a mAb, dramatically diminished IgG level produced by autologous B cells co-cultured with SSc CD4+ T cells or 5-azaC-treated CD4+ T cells but its isotype control IgG did not.

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