Figure 3
From: Transcriptional repression is epigenetically marked by H3K9 methylation during SV40 replication
The 5,6-dichloro-1-beta-D-ribofuranosylbenzimidizole (DRB)- stimulated introduction of H3K9me2 and H3K9me3 does not require the presence of H3K9me1. SV40 minichromosomes from the mutant virus cs1085 were isolated at 48 hr post-infection with or without treatment with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidizole (DRB) from 24 to 48 hr post-infection. Intact minichromosomes were subjected to ChIP analyses with antibody recognizing H3K9me2 and H3K9me3 and the intact SV40 genomic DNA present in the bound fraction quantitated by real-time PCR with primers recognizing the promoter region. The percentages of minichromosomes in the treated and untreated samples were determined and the fold increase in the percentage in the treated samples compared to the untreated calculated.