Table 1 List of sequence and product length of the real-time PCR primers used in this study
From: Expression profiling of DNA methylation-mediated epigenetic gene-silencing factors in breast cancer
Gene | Primer sequence | Product length (bp) | |
|---|---|---|---|
DNMT1 | F | 5′-GGCTGAGATGAGGCAAAAAG -3′ | 112 |
R | 5′-ACCAACTCGGTACAGGATGC -3′ | ||
DNMT3A | F | 5′-TATTGATGAGCGCACAAGAGAGC -3′ | 111 |
R | 5′-GGGTGTTCCAGGGTAACATTGAG -3′ | ||
DNMT3B | F | 5′-AATGTGAATCCAGCCAGGAAAGGC -3′ | 191 |
R | 5′-ACTGGATTACACTCCAGGAACCGT -3′ | ||
MeCP2 | F | 5′-TGACCGGGGACCCATGTAT -3′ | 145 |
R | 5′- CTCCACTTTAGAGCGAAAGGC -3′ | ||
MBD1 | F | 5′- CCTGGGTGCTGTGAGAACTGT -3′ | 107 |
R | 5′- TTGAAGGCAATTCTCTGTGCTC -3′ | ||
MBD2 | F | 5′- AGGTAGCAATGATGAGACCCTTTTA -3′ | 116 |
R | 5′- TAAGCCAAACAGCAGGGTTCTT -3′ | ||
MBD3 | F | 5′- CCGCTCTCCTTCAGTAAATGTAAC -3′ | 101 |
R | 5′- GGCTGGAGTTTGGTTTTCAGAA -3′ | ||
MBD4 | F | 5′- AGACCCGCCGAATGACCT -3′ | 144 |
R | 5′- GCACCAAACTGAGCAGAAGCG -3′ | ||
β-ACTIN | F | 5′- CTGGAACGGTGAAGGTGACA -3′ | 140 |
R | 5′- AAGGGACTTCCTGTAACAACGCA -3′ | ||