Figure 6

Microarray analysis of gene expression in AML-3 leukemic cells after treatment with 5-AZA-CdR (A), DZNep (D), TSA (T), and combinations A + D, A + T, D + T, and A + D + T. Control or untreated cells are labelled as C. The cells were treated with 50 nM 5-AZA-CdR, and, at 24 h, 1,000 nM DZNep and/or 40 nM TSA were added to the medium. At 48 h RNA was isolated for microarray analysis as described in Methods. The cluster diagram is a two-way clustering using the Ward method of the top 1,000 most up-regulated genes in response to the triple combination (A + D + T) relative to the control (C). The data plotted is log2 transformed, quantile normalized, and scaled to equal variance. The colors (blue to yellow to red) represent the spectrum of gene expression variation between conditions from low to high.