Figure 3

Cell cycle analysis of leukemic cells after sequential treatment with 5-AZA-CdR (A), DZNep (D), and/or TSA (T). AML-3 cells (A) and HL-60 cells (B) were treated with 20 nM 5-AZA-CdR and, at 24 h, 500 nM DZNep and/or 40 nM (AML-3) or 20 nM (HL-60) nM TSA were added to the medium. At 48 h cell cycle analysis was performed using flow cytometry of cells stained by propidium iodide. The results are expressed as mean ± SEM, n = 3.