Figure 1

Growth inhibition assay (A, B) and colony assay (C, D) of leukemic cells after sequential treatment with 5-AZA-CdR (A), DZNep (D), and/or TSA (T). The leukemic cells were treated with 20 nM 5-AZA-CdR and, at 24 h, 500 nM (AML-3) or 1,000 nM (HL-60) DZNep and/or 40 nM (AML-3) or 80 nM (HL-60) nM TSA were added to the medium. At 48 h the cells were counted and placed in soft agar for colony assay to determine growth inhibition and cell survival, respectively. The results are expressed as mean ± SEM, n = 3. Statistical analysis for growth inhibition and reduction in survival: A + D + T > (A + D, A + T) P A<0.05 (one way ANOVA).