Figure 1

PTEN gene hypermethylation analysis in multiple myeloma patients. (A) Representative methylation specific polymerase chain reaction (MSP) results for PTEN in patient samples. The positive and negative controls showed the expected MSP results with normal DNA showing positive U-MSP but negative M-MSP amplification, and conversely, the methylated control DNA showing negative U-MSP but positive M-MSP amplification. (B) Kaplan-Meyer survival function of multiple myeloma (MM) patients with or without PTEN promoter hypermethylation, P value = 0.53. (C) Heatmap of the PTEN analyses showing methylation status, quantitative reverse transcriptase PCR (qRT-PCR) for PTEN relative gene expression and cytogenetic categories. MSP green, unmethylated; red, methylated; qRT-PCR: green, low expression; red, high expression and white, not determined. Cytogenetics: green, standard risk; red, high risk. (D) Box and whisker plot showed the PTEN expression in methylated and unmethylated primary MM patients, P value = 0.87. (E) Survival curves of 58 patients with multiple myeloma according to SD staging (P = 0.053), and cytogenetic categories (P = 0.11).